Method for promoting hematopoietic stem cell (HSC) generation, cell composition and uses thereof

Hematopoietic Stem Cells (HSCs) are a rare population of cells which occupy the apex of the hematopoietic hierarchy, giving rise to the entire blood system throughout the whole life. Because of this crucial function, the in vitro generation of HSCs capable of full-in-vivo restoration of the blood system reamains a major challenge in the field of regenerative medicine. Several strategies to generate HSCs have been tackled during the last decades, and in vitro production of multipotent HSCs than can successfully engraft in vivo has been reported.The present invention pertains to the field of cell biology and regenerative medicine, particularly hematopoietic stem cell (HSC) technology and cell-based therapies. More specifically, the ivnetion relates to methods and compositions for obtaingin, enriching, and7or genetically modifying KDR cell pupulations, inlcuding mesodermal KDR cells and precursors thereof, for hematopoietic reconstitution, treatment of cytopenic disorders, and bone marrow regeneration in subjects affected by hematopoietic failure or undergoing myeloablative therapies.